With the development of monoclonal antibodies. They have various utilities for in-vitro diagnosis in differentiating tumor cell type, and for therapeutic purpose. I experimented the reaction of many leukemic cells. Hematopoietic and
nonhematopoietic
cells with many known and new monoclonal antibodies using flow cytometry.
@ES The results are as follows:
@EN 1) There were 18 cases of B-lineage Acute lymphoblastic leukemia(ALL) (64%), 8 cases of T-lineage ALL(28%), 1 case of B-myeloid ALL(4%) and 1case of B cell ALL(4%) in 28 cases of ALL.
2) The reactions with anti-CD9, CD10, CD19, CD24, CD34, CD72 and HLA-DR were positive in most cases of non-T-ALL. and the reaction with anti-CD13, CD33, CD40 were relatively positive in a few cases of non-T-ALL.
3) The reaction with anti-CD24, CD19, CD20. CD72 and HLA-DR was strong positive in 1 cases of Hairy cell leukemia(HCL).
4) There were 17 cases of B-Chronic lymphoytic leukemia(CLL)(71%), and 4 cases of T-CLL(17%) in 24 cases of CLL.
5) There were 8 cases of B- non Hodgkin's malignant lymphoma(NHL)(57%), and 4 cases of T-NHL(29%) in 14 cases of NHL.
6) There were positive reaction with anti-CD2 in normal bone marrow, fetal bone marrow with anti-CD19, tonsil with anti-CD4, anti-CD19, and thymus with anti-CD2.
Immunophenotype with panel of monoclonal antibodies using flow cytometry is very helpful to take diagnosis and treatment for hematologic malignancies.
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